High Performance Liquid Chromatography (HPLC) is one mode of chromatography, the most widely used analytical technique. HPLC utilises a liquid mobile phase to separate the components of a mixture. The interaction of the solute with mobile and stationary phases can be manipulated through different choices of both solvents and stationary phases. As a result, HPLC acquires a high degree of versatility not found in other chromatographic systems and it has the ability to easily separate a wide variety of chemical mixtures.

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